DNA sequence analysis with droplet-based microfluidics
نویسندگان
چکیده
منابع مشابه
Droplet microfluidics.
Droplet-based microfluidic systems have been shown to be compatible with many chemical and biological reagents and capable of performing a variety of "digital fluidic" operations that can be rendered programmable and reconfigurable. This platform has dimensional scaling benefits that have enabled controlled and rapid mixing of fluids in the droplet reactors, resulting in decreased reaction time...
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Microfluidics allows the manipulation of small quantities of reagents in a high-throughput manner and is therefore highly amenable to single cell characterization and more generally to digital analysis, with applications in fields as varied as genomics, diagnostics, directed evolution, and drug screening. The growing place of microfluidics in biology laboratories encouraged us to develop a ...
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The feasibility of implementing pyrosequencing chemistry within droplets using electrowetting-based digital microfluidics is reported. An array of electrodes patterned on a printed-circuit board was used to control the formation, transportation, merging, mixing, and splitting of submicroliter-sized droplets contained within an oil-filled chamber. A three-enzyme pyrosequencing protocol was imple...
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In this paper, we develop a strategy to form on-demand droplets with specific pH values. The pH control is based on electrolysis of water in microfluidics, and the produced hydrogen and hydroxyl ions are separated and confined in individual containers during the droplet generation, triggered by a pressure pulse. By tuning the applied voltages and pressure pulses, we can control on demand the pH...
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Droplet microfluidics (DMF) is a fluidic handling technology that enables precision control over dispensing and subsequent manipulation of droplets in the volume range of microliters to picoliters, on a micro-fabricated device. There are several different droplet actuation methods, all of which can generate external stimuli, to either actively or passively control the shape and positioning of f...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2013
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c3lc50905b